e hsel r0001  (Elabscience Biotechnology)

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits NF-κB signaling pathway activation and inflammatory factor secretion in SCs. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in SCs after CXCL12 treatment. ( B - C ) Quantification of gray values for the NF-κB, p-NF-κB, κBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in SCs after CXCL12 treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits the inflammatory response in SCs via the NF-κB signaling pathway. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in SCs after NF-κB overexpression. ( B - C ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in SCs after NF-κB overexpression. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing, Over Expression

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits SC ferroptosis, thereby mitigating the inflammatory response. ( A ) Western blot analysis of the expression levels of ferroptosis-related proteins ACSL4, GPX4, and FSP1 in SCs after FAC treatment. ( B - D ) Densitometric analysis of proteins ACSL4, GPX4, and FSP1. ( E , F ) Fluorescence images of ROS and Fe 2+ in SCs after FAC treatment, Scale bar = 50µm. ( G , H ) Quantification of the relative fluorescence intensity of ROS and Fe 2+ . ( I , J ) Expression levels of MDA and GSH in SCs after FAC treatment. ( K ) Western blot analysis of the expression levels of NF-κB, p-NF-κB, IκBα, and p-IκBα in SCs after FAC treatment. ( L , M ) Densitometric analysis of proteins NF-κB, p-NF-κB, IκBα, and p-IκBα. ( N , O ) Expression levels of IL-1β and TNF-α in SCs after FAC treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing, Fluorescence

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits NF-κB signaling pathway activation and inflammatory factor secretion in the sciatic nerve. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in the sciatic nerve after CXCL12 treatment. ( B - C ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in the sciatic nerve after CXCL12 treatment. The data are presented as the means ± SDs, n=3, **p < 0.01, ***p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits sciatic nerve ferroptosis, thereby mitigating the inflammatory response. ( A ) Western blot analysis of ferroptosis-related protein expression levels (ACSL4, GPX4, FSP1) in the sciatic nerve after FAC treatment. ( B - D ) Quantification of the gray values for the ACSL4, GPX4, and FSP1 proteins. ( E ‒ G ) Levels of Fe 2+ , MDA, and GSH in the sciatic nerve after FAC treatment. ( H ) Western blot analysis of NF-κB, p-NF-κB, IκBα and p-IκBα protein expression levels in the sciatic nerve after FAC treatment. ( I , J ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα and p-IκBα proteins. (K, L) Levels of IL-1β and TNF-α in the sciatic nerve after FAC treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing

Journal: Journal of Microbiology and Biotechnology

Article Title: Effects of Acute Abdomen III on Sepsis-Induced Intestinal Damage in a Rat Model

doi: 10.4014/jmb.2412.12067

Figure Lengend Snippet: Levels of serum interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) in sepsis-induced rats treated with or without acute abdomen III were measured using enzyme-linked immunosorbent assays (ELISA). ( B ) Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) content in intestinal tissue was determined using spectrophotometry, and corresponding detection kits. ( C ) Endotoxin levels in serum, D-lactate levels, and diamine oxidase (DAO) levels were evaluated using specific test kits in sepsis-induced rats treated with or without acute abdomen III. ** P < 0.01; *** P < 0.001. Each group consisted of 3 rats.

Article Snippet: Serum samples were obtained by centrifuging blood samples at 3,000 × g for 15 min at 4°C, in which TNF-α, IL-6, IL-1β, and diamine oxidase (DAO) levels were measured using ELISA kits (E-HSEL-R0001; E-EL-R0015; E-EL-R0012; E-EL-R3013; Elabscience Biotechnology Inc., China).

Techniques: Enzyme-linked Immunosorbent Assay, Spectrophotometry